polyA+ RNA-Seq of C57BL/6xCBA Thumpd2 knockout mice - SRA

SRX24648884: polyA+ RNA-Seq of C57BL/6xCBA Thumpd2 knockout mice
1 ILLUMINA (Illumina HiSeq 1500) run: 55.7M spots, 6.3G bases, 1.6Gb downloads

Design: Mice line with Thumpd2 gene knockout was created by microinjection of Cas9 mRNA along with sgRNA targeting Thumpd2 gene into C57BL/6xCBA mice zygotes. Thumpd2+/- mice were back-crossed to the C57BL/6 genetic background and mated to obtain homozygous viable Thumpd2-/- mice. Total RNA from the mouse liver samples was extracted using QIAzol lysis reagent. NEB NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770) was used for library preparation.

Submitted by: Lomonosov Moscow State University

Study: Study of the splicing in Thumpd2 knockout mice

PRJNA1114654 • SRP509174 • All experiments • All runs

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Splicing is an important step in the maturation of most eukaryotic RNAs. In this work, we studied the effect of m2G72 modification in U6 snRNA on splicing in mouse liver. We performed high-throughput polyA+ RNA sequencing of liver samples from wild-type and Thumpd2 knockout mice. Thumpd2 is methyltransferase catalyzing the indicated N2-methylation of U6 snRNA. Three biological replicates were made for each type of mouse.

Sample:

SAMN41489823 • SRS21385687 • All experiments • All runs

Organism: Mus musculus

Library:

Name: deltaTh2-2

Instrument: Illumina HiSeq 1500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: Oligo-dT

Layout: SINGLE

Runs: 1 run, 55.7M spots, 6.3G bases, 1.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR29125084	55,678,072	6.3G	1.6Gb	2024-05-22

ID:: 32967764

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